ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2009, Vol. 1 ›› Issue (1): 51-53.doi: 10.3969/j.issn.1674-3865.2009.01.016

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清肺口服液含药血清对呼吸道合胞病毒黏附、膜融合影响的实验研究

袁斌，廖辉，汪受传，徐建亚，李华丽，李江全   

  1. 210029 南京，南京中医药大学附属医院儿科(袁斌)；南京中医药大学儿科教研室(廖辉，汪受传，徐建亚，李华丽，李江全)
  • 收稿日期:2008-07-24 出版日期:2009-02-25 上线日期:2009-02-25
  • 通讯作者: 袁斌，E-mail：yuanbin68358@163.com
  • 作者简介:袁斌(1966-)，男，医学博士，副教授、副主任医师，硕士研究生导师。擅长肺系疾病与肾系疾病的诊断和治疗，E-mail：yuanbin68358@163.com。
  • 基金资助:
    江苏省自然科学基金项目（BK2005219）

Experimental study on influence of  Qingfei oral liquid medicine contained serum on adhesion and membrane fusion process of respiratory syncytial virus

YUAN Bin，LIAO Hui，WANG Shou chuan   

  1. Department of Pediatrics,The Affiliated Hospital to Nanjing University of TCM，Nanjing 210029，China
  • Received:2008-07-24 Published:2009-02-25 Online:2009-02-25

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摘要： 目的:研究清肺口服液含药血清对呼吸道合胞病毒黏附、膜融合过程的抑制作用。方法:应用温度转换的方法，在病毒黏附阶段（温度转换前）和病毒侵入阶段（温度转换后）分别加入清肺口服液含药血清，监测两个阶段病毒抑制率和平均吸光度(A)值，观察研究清肺口服液含药血清对呼吸道合胞病毒黏附和膜融合的影响。结果:温度转换实验前，含药血清组平均A值与病毒对照组无显著性差异；病毒侵入阶段（温度转换后）的清肺口服液含药血清组病毒抑制率为增多，平均A值明显升高。温度转换实验前后差异有统计学意义。结论:清肺口服液含药血清的抗病毒作用主要表现在呼吸道合胞病毒黏附后的膜融合及侵入阶段。

关键词: 清肺口服液/血清学, 呼吸道合胞病毒/中医药疗法, 清热解毒剂, 实验

Abstract: Objective:To study the inhibition effects of Qingfei oral liquid medicine-contained serum on adhesion and membrane fusion process of respiratory syncytial virus.Methods:Using the method of temperature conversion，we added Qingfei oral liquid medicine-contained serum during adhesion period(before temperature conversion) and invasion period(after temperature conversion) respectively.Monitor virus inhibition rates and average A of the two periods，observe the influence of Qingfei oral liquid medicine-contained serum on adhesion and membrane fusion process of respiratory syncytial virus.Results:No significant differences were found on the average A between Qingfei oral liquid medicine-contained serum group and virus control group before temperature conversion experiment.After the temperature conversion，virus inhibition rates of Qingfei oral liquid medicine-contained serum group increased，the average A increasing obviously.Differences were significant before and after temperature conversion experiment.Conclusion:The antiviral effect of Qingfei oral liquid medicine-contained serum mainly embodies on the membrane fusion and invasion phases  after adhesion process of respiratory syncytial virus.

Key words: Qingfei oral liquid/Serology；RSV/TCM therapy；Heat-clearing and detoxifying agents, Expertimental