ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2011, Vol. 3 ›› Issue (1): 32-35.doi: 10.3969/j.issn.1674-3865.2011.01.015

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丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制研究

牟德英,刘小凡   

  1. 611130 成都，成都市温江区中医医院儿科；610075 成都，成都中医药大学附属医院儿科（刘小凡）
  • 收稿日期:2010-09-27 出版日期:2011-02-25 上线日期:2011-02-25
  • 通讯作者: 刘小凡
  • 作者简介:牟德英（1976-），女，医学硕士，主治医师。研究方向：小儿呼吸系统疾病的诊断和治疗。

The research of effectiveness and mechanism of Danlong panting-arresting decoction in airway remodeling in asthmatic guinea pig

MOU De ying，LIU Xiao fan   

  1. TCM Hospital of Wenjiang，Chengdu 611130，China
  • Received:2010-09-27 Published:2011-02-25 Online:2011-02-25

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摘要： 目的：初步探析丹龙定喘汤对哮喘豚鼠气道重塑的作用及机制。方法：〗选取健康雌性豚鼠24只，按体质量分层随机分为4组：空白对照组、模型对照组、地塞米松组、丹龙定喘汤组，每组6只。采用卵蛋白致敏法建立豚鼠哮喘气道重塑模型，以丹龙定喘汤干预，并以地塞米松做对照药物，治疗1个月后分别检测气道重塑的程度及豚鼠肺组织中转化生长因子β1（TGF-β1)含量。结果：(1)模型对照组豚鼠肺支气管管壁和平滑肌层明显增厚，与空白对照组相比差异有统计学意义（P<0.05）;丹龙定喘汤组气道壁厚度减轻，与模型对照组相比差异有统计学意义（P<0.05），与地塞米松组相比，差异无统计学意义(P＞0.05)。(2)模型对照组豚鼠肺组织中的TGF-β1含量明显增加，与空白对照组相比，差异有统计学意义（P<0.05）；丹龙定喘汤组TGF-β1含量减少，与模型对照组相比差异有统计学意义（P<0.05），与地塞米松组相比差异无统计学意义（P＞0.05）。结论：丹龙定喘汤对哮喘豚鼠气道重塑有一定抑制作用，其机制可能是通过下调哮喘豚鼠肺组织中的TGF-β1水平,抑制气道平滑肌细胞及细胞外基质的增殖而阻断气道重塑的进程。

关键词: 哮喘；祛痰/中医药疗法；丹龙定喘汤/治疗应用；气道重塑;豚鼠；动物, 实验

Abstract: Objective：To make a preliminary exploration of the effectiveness and mechanism of Danlong panting-arresting decoction in airway remodeling in asthmatic guinea pig.Methods：Twenty-four healthy female guinea pigs were divided into four groups randomly（n=6，in each group）：normal control group, model control group，dexamethasone group，and Danlong panting-arresting decoction group.The asthmatic guinea pig airway remodeling model was established by ovalbumin sensitized method,Danlong panting-arresting decoction was applied to intervene，and dexamethasone was used as control drug.After one month,detect the extent of airway remodeling and determine TGF-β1 level in guinea pig lung tissues.Results：（1）Compared with normal group，WAt/Pi，WAm/Pi were significantly increased(P<0.05) in model group.Compared with model group，the airway wall thickness reduced in Danlong panting-arresting decoction group，there being a significant difference(P<0.05),but no significant difference compared with dexamethasone group(P>0.05).(2)Compared with normal group，the extent of lung tissue TGF-β1 was significantly increased in the model group(P<0.05).Compared with model group，the TGF-β1 content was decreased in Danlong panting-arresting decoction group，there being a significant difference(P<0.05),but no significant difference compared with the dexamethasone group(P>0.05).Conclusions：Danlong panting-arresting decoction plays an inhibitory role in airway remodeling of asthmatic guinea pigs.It may reduce the TGF-β1 level of lung tissues in asthmatic guinea pigs，thus inhibit airway proliferation of smooth muscle cells and extracellular matrix and block the airway remodeling process.

Key words: Asthma；Resolving phlegm/TCM therapy；Danlong panting-arresting decoction；Airway remodeling；Guinea pig；Animal, experiment