Abstract:
Objective
To explore the significances of detection of intercellular adhesion molecule-1(ICAM-1) in peripheral blood and regulated upon activation normal T cell expressed and secreted(RANTES) in bronchoalveolar lavage fluid in children with lobar pneumonia and their correlation in pathogenesis of lobar pneumonia.
Methods
A total of 95 children with respiratory diseases were included  as the research subjects, and they were divided into 3 groups: lobar pneumonia group(35 cases),bronchopneumonia group(30 cases) and tracheal stenosis group(control group,30 cases). The expression of ICAM-1 was detected by flow cytometry in lymphocyte of peripheral blood in 35 children with lobar pneumonia and 30 children with bronchopneumonia and 30 children with tracheal stenosis, and the level of RANTES was assayed with ELISA technique in the bronchoalveolar lavage fluid.
Results
(1)The expression rate of ICAM-1 in lobar pneumonia group was much higher than that in bronchopneumonia group（P<0.05） and control group（P<0.01）,and it was higher in bronchopneumonia group than in control group（P<0.05）.(2)The level of RANTES in lobar pneumonia group and bronchopneumonia group was higher than that in control group（P<0.05）;however，no significant difference was found between lobar pneumonia group and bronchopneumonia group.(3)There was a positive correlation between ICAM1 and RANTES in lobar pneumonia group(r=0.781，P＜0.01); however，no correlation was found in bronchopneumonia group and control group.
Conclusion
ICAM-1 and RANTES are involved in the development of lobar pneumonia and play a synergistic role in it, so they can be used as indicators of lobar pneumonia, which provides theoretical basis for studying the pathogenesis of lobar pneumonia.

Key words: Lobar pneumonia, Intercellular adhesion molecule-1, Regulated upon activation normal T cell expressed and secreted, Flow cytometry, Bronchoalveolar lavage fluid, Child