ObjectiveTo observe the blocking and inhibiting effect of streamlined formula of Yinqiao powder (SFYQP) and Yupingfeng powder (YPFP) extract on influenzaA FM1 strains in chicken embryo.

MethodsChicken embryo experiment technology was used to observe the effectiveness of SFYQP and YPFP extract on inhibiting influenza A virus (IV) FM1 strains.
ResultsED50 of IV infected chicken embryo was 10-6. Themaximum of no toxicity concentration (TC0) of SFYQP and YPFP extract was 39.15 g/L and 36.2 g/L,respectively. The TC0 of ribavirin was 100 g/L. SFYQP extract had effect on preventing infection by IV FM1 strains. The ED50 was 7.41 g/L, and its therapeutic index (TI) was more than 5.28. When its concentration was 19.58 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05).SFYQP extract had effect of inhibiting infection by IV FM1 strains. Its ED50 was 9.91 g/L, and its TI was more than 3.95. When its concentration was 39.15 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05). YPFP extract also had effect of preventing infection by IV FM1 strains. Its ED50 was 9.57 g/L, and its TI was more than 3.78. When its concentration was 36.2 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration (P＜0.05).YPFP extract had the effect of inhibiting infection by IV FM1 strains. Its ED50 was 10.38 g/L, and its TI was more than 3.3. When its concentration were 39.15 g/L and 18.1 g/L, no infection rate on two concentrations was both 83%, and their effect was better than on the concentration of 9.05 g/L (P＜0.05). On observing the death blocking of  extract on chicken embryos, the blocking of YPFP extract was more effective than SFYQP extract, though, inhibition of SFYQP extract was more effective than YPFP extract. Moreover,blocking and inhibition of both extracts were obviously better than ribavirin (P＜0.05).

ConclusionSFYQP and YPFP extracts have the effect of blocking and inhibition on IV FM1 strains infected chicken embryo.