Abstract:
【Abstract】Objective：To observe the effect and the mechanism of Qingzao Jiufei decoction on pathological inflammation of lung tissue and bronchoalveolar lavage fluid (BALF) in rats infected with mycoplasma pneumoniae (MP).
Methods：Totally 90 rats were randomized into 6 groups, namely, normal group (group A), model group (group B), Qingzao Jiufei highdose group (group C), Qingzao Jiufei mediumdose group (group D), Qingzao Jiufei lowdose group (group E) and roxithromycin group (group F) ,with 15 rats in each. The rats of the last five groups were made into MP infection models. The same volume of drugs or normal saline was given to each group by introgastric administration respectively. The pathological inflammation score of lung tissue, MP and MPDNA of BALF was detected after 7, 10 and 14 days of MP infection. MP of BALF was tested by polymerase chain reaction (PCR) test, and MPDNA content was tested by fluorescent quantitative PCR (Realtime FQ PCR).
Results：Seven days after MP infection models were made, the pathological changes, the positive rate and the content of MPDNA in B group were obviously higher than those in group A, which showed that MP infection models were made successfully. In group C and group F pulmonary inflammation had obvious improvement; group D achieved effective results after 10 and 14 days of infection; group E did not achieve obvious effect. Group C, D and F had significantly more effect than other groups in reducing MPDNA in BALF; the effect of group E was not obvious at 7th and 10th day, but had an advantage at 14th day.
Conclusions：It shows that Qingzao Jiufei decoction is effective in antiMP, and the efficacy might be correlated with dose and time of administration and infection.