miRNAs小鼠哮喘模型中的作用初探

Abstract

Abstract: Objective：To investigate the differential expression of miRNAs in asthma model of mice.
Methods：BALB/c mice were randomly divided into normal control group， asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group)， 8 mice in each group. Eosinophil(EOS)， neutrophil(NEU)， macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4，IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 was statistically analyzed.
Results：The expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 in the asthma group was significantly higher than that in the control group, and the expression of various factors was downregulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed，and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13.
Conclusion：miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

Key words: Asthma, MicroRNA, Inflammatory factor, Cytokine

ZHANG Dongjiao，XU Hao. Role of miRNAs in the model of asthma in mice[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(1): 1-.

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Role of miRNAs in the model of asthma in mice

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