Abstract: Objective〖WT5"BZ〗Observe the effects on TNFα and ET1 expression in renal tissues of rats with mesangial proliferative glomerulonephritis(MsPGN) by Chinese herbs with functions of both the heatclearing and detoxification(HD,Qingre Jiedu),and the removing of blood stasis and channel obstruction(RBSCO,Huayu Tongluo),or each of them,in order to explore mechanism of the Chinese herbs.
〖WT5"HZ〗Methods〖WT5"BZ〗Male SD rats were included as the study object.The rat models of MsPGN were made by taking orally and timecontrolled vein injecting boving serum albumin(BSA),and vein injecting staphylococcus enterotoxin B(SEB),and spot of division of hypodermic injecting complete Freund's adjuvant and not complete Freund's adjuvant.The proliferation of glomerular mesangial cell(MC) and glomerular extracellular matrix(ECM) was studied by light microscope on rat models of MsPGN,who were treated by combined HD with RBSCO,and by HD,or by RBSCO，respectively.TNFα and ET1 expression in renal tissues was detected by immunohistochemical method,and quantitative analysis was made.
〖WT5"HZ〗Results〖WT5"BZ〗In the successful mesangial proliferative nephritis models,glomerular mesangial proliferation was mild to moderate,and TNFα and ET1 expression in renal tissues increased significantly(P＜0.01).The treatment of combination of HD with RBSCO could significantly reduce TNFα and ET1 expression levels in the renal tissues.HD or RBSCO respectively might have different action mechanism,because HD group was emphased on reducing TNFα expression(P＜0.01),while RBSCO Group was emphased on reducing ET1 expression(P＜0.01).
〖WT5"HZ〗Conclusions〖WT5"BZ〗The treatment of combination of HD with RBSCO for MsPGN is more effective than that of single HD or RBSCO,and it can reduce the renal pathological damage through different treatment mechanisms.

Key words: MsPGN/The therapy of TCM；Mesangial proliferative；TNFα；ET1；Rat；Animal, experiment