miRNAs小鼠哮喘模型中的作用初探

doi:10.3969/j.issn.1674-3865.2021.01.001

Abstract

Abstract: Objective：To investigate the differential expression of miRNAs in asthma model of mice.Methods：BALB/c mice were randomly divided into normal control group， asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group)， 8 mice in each group. Eosinophil(EOS)， neutrophil(NEU)， macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4，IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 was statistically analyzed.Results：The expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13 in the asthma group was significantly higher than that in the control group, and the expression of various factors was down regulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed，and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS，NEU，MAC，LYM，IL-4，IL-6 and IL-13.Conclusion：miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

Key words: Asthma, MicroRNA, Inflammatory factor, Cytokine

ZHANG Dongjiao, XU Hao. Role of miRNAs in the model of asthma in mice[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(1): 1-.

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Role of miRNAs in the model of asthma in mice

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