ISSN 1674-3865  CN 21-1569/R
主管:国家卫生和计划生育委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

Chinese Pediatrics of Integrated Traditional and Western Medicine ›› 2019, Vol. 11 ›› Issue (2): 139-143.doi: 10.3969/j.issn.1674-3865.2019.02.013

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Inhibitory effect of Xiao'er Zidian Zhenxiao granule on inflammation of human umbilical vein endothelial cells induced by serum IgA1 in children with HenochSchonlein purpura and its mechanism

ZHU Haoyu,DUAN Xiaozheng,FENG Xiaochun   

  1. Children's Medical Center,Affiliated Hospital of Changchun University of Traditional Chinese Medicine,Changchun 130021,China
  • Online:2019-04-25 Published:2019-05-08

Abstract:
Objective
To investigate the inhibitory effect of Xiao'er Zidian Zhenxiao granules on the inflammation of human umbilical venous endothelial cells(HUVECs) induced by serum IgA1 in children with HenochSchnlein purpura(HSP), and to clarify its mechanism.
Methods
HUVECs were used as the research subjects, serum IgA1 in HSP children and in healthy children were used to intervene, and different concentrations of Xiao'er Zidian Zhenxiao granules were added. They were divided into blank control group, normal control group, Henoch-Schonlein purpura group(HSP group) and the Xiao'er Zidian Zhenxiao granules groups(ZXKL groups)(the low-,medium-and high-concentration group, with the concentration being 25 mg/L, 50 mg/L and 100 mg/L respectively). After 24 hours of culturing in each group, TNF-α and IL-8 levels in the supernatant of each group were determined by enzyme-linked immunosorbent assay. Westernbolt was used to detect the expression of NF-κB protein in each group.
Results
The levels of TNF-α and IL-8 in the supernatant of each group were significantly higher in the HSP group than in the blank and normal control groups(P<0.001). Compared with the HSP group, the levels of TNF-α and IL-8 in the ZXKL groups decreased(P<0.05). The expression of P65 protein in HUVECs was detected by Western blot. Compared with the blank and normal control groups, the P65 protein level in the HSP group was significantly increased(P<0.05). Compared with the HSP group, the P65 protein level in the high-concentration ZXKL group(100 mg/L) was decreased(P<0.05).
Conclusion
Xiao'er Zidian Zhenxiao granules can decrease the phosphorylation of NF-κB signaling pathway P65 protein in HUVECs and reduce the secretion of related inflammatory cytokines(TNF-α and IL-8), thus effectively inhibiting the occurrence of cellular inflammatory responses.

Key words: Henoch-Schonlein purpura, Xiao'er Zidian Zhenxiao granules, Human umbilical venous endothelial cell, Nuclear factor-κB