Abstract: Objective
To observe the effect of Yiqi Jiedu Huayu Xiao Fufang and its single drug on proliferation and apoptosis of glomerular mesangial cells in rats and explore its mechanism.
Methods
A total of 60 Wistar rats were randomly divided into normal group, Xiaofufang group, Huangqi group, Danshen group, Baihuasheshecao group and Telmisartan group, with 10 rats in each group. The normal group was given 4 mL of distilled water daily, and each drug group was given 2 mL of drug in the morning and evening for 3 consecutive days. Rat mesangial cells were routinely cultured, and the cultured mesangial cells were randomly divided into normal group, Xiaofufang group, Huangqi group, Danshen group, Baihuasheshecao group, Telmisartan group, and Angiotensin Ⅱ group. Angiotensin Ⅱ 1×10-6mol/L was added to each group. The proliferation of rat mesangial cells was detected by MTT colorimetric assay. The apoptosis of rat mesangial cells was determined by flow cytometry.
Results
Compared with Angiotensin II group, other drug intervention groups had effect of inhibiting the proliferation and increasing apoptosis rate of mesangial cells(P<0.01 or P<0.05); there was no significant difference among the other groups(P>0.05). Compared with other drug intervention groups, the Xiaofufang group had the strongest inhibition and apoptosis of mesangial cells(P<0.01 or P<0.05); there was no significant difference among the other groups(P>0.05).
Conclusion
Yiqi Jiedu Huayu Xiao Fufang and its single drug can inhibit the proliferation and apoptosis of rat mesangial cells, which can slow the development of glomerular sclerosis, and the effect of Yiqi Jiedu Huayu Xiao Fufang is the best.

Key words: Mesangial cells, Yiqi Jiedu Huayu Xiao Fufang, Cell proliferation, Apoptosis