Abstract: Objective To detect the expression and significance of Toll-like receptor 2/4（TLR2/4） in peripheral blood monocytes of children with rotavirus（RV） enteritis complicated with myocardial damage.Methods Totally 25 cases of RV enteritis with myocardial damage and 25 cases of RV without myocardial damage were selected as the research subjects，another 25 healthy children were selected as the control group. Serum CK，CK-MB，LDH，AST，TNF-α，IL-8，CRP and INF-γ were detected by enzyme-linked immunosorbent assay（ELISA），TLR2/4 gene expression in peripheral blood monocytes of patients in each group was detected by real-time fluorescence quantitative PCR（RT-qPCR），the expression of TLR2/4 protein in peripheral blood monocytes was detected by flow cytometry.Results The serum CK，CK-MB，LDH，AST，TNF-α，IL-8，CRP，INF-γ and the gene and protein expression of TLR2 and TLR4 in peripheral blood monocytes in the observation group and control group were higher than those in the healthy group（P＜0.05），and they were higher in the observation group than in the control group（P＜0.05）.Conclusion The levels of TLR2 and TLR4 in peripheral blood monocytes of children with RV enteritis complicated with myocardial damageare significantly higher than those of children in control group and healthy group，which can be used as a diagnostic index for early diagnosis of RV enteritis complicated with myocardial damage.

Key words:

Rotavirus, Enteritis, Myocardial damage, TLR2, TLR4