|
|
The Intervention of Xifeng capsule on spontaneous recurrent seizures and hippocampalformation lesion in epileptic rats induced by Lithium-Pilocarpine
Li Xinmin, Qin Luping, Lu Yanli
2012, 4 (6):
481-484.
doi: 10.3969/j.issn.1674-3865.2013.02.001
Objective：〖WT5"BZ〗 To study the intervention of Xifeng capsule single and combination effect on spontaneous recurrent seizures(SRS) and hippocampal formation lesion in epileptic rats induced by Lithiu-Pilocarpine.〖WT5"HZ〗Methods：〖WT5"BZ〗 All experimental rats were randomly divided into eight groups: normal control group, model group, Xifeng high-dose group, Xifeng middle-dose group, Xifeng low-dose group, Xifeng high-dose + TPM group, Xifeng high-dose + TPM 1/2 dose group, TPM group. We use lithium-pilocarpine chemical ignition method to duplicate the epileptic rat model.And with conventional histopathological method and transmission electron microscope were utilized to observe morphological changes in hippocampal formation,Timm histochemical technique was adopted to study mossy fiber sprouting.〖WT5"HZ〗Results：〖WT5"BZ〗(1)To compare the situation from the seizures in rats, the average attack level of all rats of Intervention group lower than model group（P-0.05）, but higher than the control group (P-0.01).(2) All intervention group, the SRS frequencies obviously lower than model group（P-0.05）.(3)In all intervention group, the total aera of mossy fiber sprouting(MFS) of the hippocampal CA3 subfield and dentate gyrus were more than that of normal group(P-0.05). Both in the hippocampal CA3 subfield and dentate gyrus, the total aera of mossy fiber sprouting(MFS) have significance difference between Xifeng high-dose group and Xifeng low-dose group（P-0.05）.(4)Using conventional histopathological method and transmission electron microscope, ultrastructure of neurons in hippocampus was regular in normal group but damaged obviously in model group.In all therapy groups. The damage of neuron in hippocampus was ameliorated in Xifeng high-dose + TPM1/2 dose group, in Xifeng high-dose group. The damage of neuron in hippocampus was less than in Xifeng middle-dose group and in Xifeng low-dose group.〖WT5"HZ〗Conclusion：〖WT5"BZ〗 The intervention of Xifeng capsule single and combination can effectively reduce the degree of epileptic seizures in rats,protect hippocampal neurons from being more seriously and deterred hippocampal mossy fiber sprouting(MFS).
Related Articles |
Metrics
|
