Abstract:

Objective To study the metabolomic characteristics of lipids and amino acids of children with respiratory syncytial virus(RSV) pneumonia with wind-heat depressing lung syndrome by liquid chromatography-mass spectrometry (LC-MS) technology.Methods A total of 30 children with RSV pneumonia with wind-heat depressing lung syndrome and 30 normal children in the same period were selected.LC-MS technology was used to detect the serum of the subjects,and the differential metabolites in lipids and amino acids in children with RSV pneumonia with wind-heat depressing lung syndrome were screened out.Results The serum metabolites of the two groups of children were significantly different,and a total of 5 differential metabolites with the variable importance value of the principal component >2 were screened out,among which phosphatidylcholine(PC 34∶3) and phenylalanine(Phe-Phe) were significantly lower in RSV children than in normal children,while sphingomyelin(SM36∶1,SM34∶0) and tryptophan(Tryptophan) were significantly higher than in normal children,there being statistically significant differences(P<0.05).Conclusion The metabolomic method based on LC-MS can distinguish children with RSV pneumonia with wind-heat depressing lung syndrome from normal children,and the screened differential metabolites may be potential diagnostic markers for children with RSV pneumonia with wind-heat depressing lung syndrome.

Key words:

Respiratory syncytial virus pneumonia；Wind-heat depressing lung syndrome；Metabonomics；Children

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