Abstract: ObjectiveTo study the effects of twostep sequential therapy on signal pathway of NFκB of airway inflammation in asthmatic mice.
MethodsForty healthy female Kunming mice were divided into four groups randomly(n=10,in each group):normal control group,asthma group,budesonide group and twostep sequential therapy group.The asthmatic models were established by ovalbumin,and budesonide and twostep sequential therapy were applied to them.Then the pathologic changes in airway tissues and bronchoalveolar lavage fluid(BALF) in cytology were observed.The level of nuclear factorκB(NFκB) and GATA3 in airway tissues was detected by immunohistochemical method.The level of interleukin4（IL4） in BALF was detected by enzymelinked immunosorbent assay(ELISA).
ResultsThe treatment groups had less inflammatory cell infiltration than asthmatic group(P<0.01),and more than normal control group(P<0.01).There was no significant difference between the budesonide group and twostep sequential therapy group(P>0.05).The level of NFκB,GATA3 and IL4 decreased in the budesonide group and twostep sequential therapy group compared with the asthmatic group(P<0.01),but increased compared with the normal control group(P<0.01).There was no significant difference between budesonide group and twostep sequential therapy group(P>0.05).
ConclusionsThe twostep sequential therapy may inhibit the activity of the signal transduction pathway of NFκB,which can reduce the expression of GATA3 and class cytokines of Th2,hence plays an important role in treating asthmatic mice．

Key words: Asthmatic；Ginsengschisandrae decoction;Minor greenblue dragon decoction;Nuclear factorκB;GATA3;Interleukin4；Mice；Animal, experiment