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25 February 2010, Volume 2 Issue 1 Previous Issue    Next Issue

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The effect of early intervention on NSCs proliferation in brain damage rats after intrauterine infection LI Xiaojie，WU Qiong. 2010, 2 (1):  4-7.  doi: 10.3969/j.issn.1674-3865.2010.01.003 Abstract ( 179 )   PDF (479KB) ( 101 )   Save ObjectiveTo study the impact of early intervention on proliferation of neural stem cells in brain damage rats after intrauterine infection. MethodsTotally 36 Wistar pregnant rats were randomly divided into two groups:LPS(n=30) and saline(n=6).Pregnant rats were consecutively injected with LPS(450 μg/kg) or saline on gestation 17 d.Saline neonatal rats were randomly selected as saline group N(n=30),LPS neonatal rat as training group T(n=30) and injury group I(n=30).The 1 d T group rats were interfered by early interventiont.At the same time,the N,I group rats were fed in the routine way.The expression of bromodeoxyuridind(BrdU) were determined by immunohistochemistry at 1，7，14，28 d of all groups.BrdU was used to mark dividing neural stem cells（NSCs) in hippocamp dentate gyrus subgranular zone（SGZ). Results（1）BrdUlabeled cells could be observed in all groups；I groups and T groups were markedly less than N group（P<0.01）.(2)The number of positive cells of N group increased at 3 day（P<0.05）,approximately six fold with a peak at 7 day,markedly decreased at 28 day and approched normal level of mature rats.(3)Compared with the N group,the number of positive cells of T group increased strikingly at 3 day（P<0.01）,exceeding N group and approximately six fold with a peak at 14 day,then gradually decreased,but still higher than N group at 28 day.The appearance of increasing peak in T group was lengthened，value ascended. ConclusionsOur results indicate that the brain damage caused by intrauterine infection can lead to the decrease of proliferation of inherent NSCs.The damage will not recover itself.Early intervention can stimulate the proliferation of inherent NSCs.Early period enriched environment can prolong the development window phase of inherent NSCs. Related Articles | Metrics

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Experimental study of the early behavior therapy to reduce cerebral palsy of the young rats due to brain damage PANG Wei，LI Xiaojie，ZHANG Shiling. 2010, 2 (1):  8-12.  doi: 10.3969/j.issn.1674-3865.2010.01.004 Abstract ( 486 )   PDF (484KB) ( 98 )   Save ObjectiveTo study the effect of early behavior therapy to reduce cerebral palsy caused by brain damage in rats. MethodsTotally 27 pregnant rats were consecutively injected with LPS(450 μg/kg，n=21) or saline(n=6) on gestation 18 d and 19 d.We selected randomly saline neonatal rat group B(n=30) and LPS neonatal rat group A1(n=20)，A2(n=30).The 2 d rats(A1) were given early behavior therapy，such as enriched environment and the rats(A2 and B) were routinely fed.The neurobehavior of the rats was observed in A1，A2 and B groups on 25 d.The MBP and S100 were detected in brain sections on 1 d and 25 d. ResultsMBP expression was obviously decreased in A2(1 d),compared with B of 1 d rats(P<0.01);MBP was increased in A1 of 25 d,compared with A2 of 25 d(P<0.05)；it was increased in B,contrasted with A1 and A2(P<0.01).Compared with B,there was significant increase in A2 of 1 d with regard to S100(P<0.01).The result of S100 between A2 and B of 25 d was the same with 1 d rats,but the S100 of A1 was the middle of them（P<0.01）．The result of neurobehavioral observation indicated that there were 4 cerebral palsy rats in A1,8 cerebral palsy rats in A2 and no cerebral palsy rat in B. ConclusionMBP and S100 can become the sign of early diagnosis and the therapeutic effect in brain injury.Early interference can reduce cerebral palsy. Related Articles | Metrics

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Preliminary investigation of relationship between oxytocin receptor gene and childhood autism JIANG Zhimei,ZHANG Lihua,LV Zhihai,et al 2010, 2 (1):  12-14.  doi: 10.3969/j.issn.1674-3865.2010.01.005 Abstract ( 337 )   PDF (303KB) ( 110 )   Save ObjectiveTo investigate the relationship between oxytocin receptor（OXTR）gene polymorphism and childhood autism in Han population in Jiamusi. MethodsPolymerase chain reactionrestricted fragment length polymorphism(PCRRFLP) was used to determine allele and genotype of SNP(rs2254298) of OXTR in 30 Chinese Han autism children staying in the 3rd Affiliated Hospital of Jiamusi University and 30 normal children in a kindergarten and an elementary school in Jiamusi from May to December,2007. ResultsThe genotype distribution of the SNP(rs2254298) in patient group and control group was consistent with the HardyWeinberg equilibrium(P>0.05).There was significant difference in the distribution of the allelic frequencie and genotype in rs2254298 between the two groups(P＜0.05). ConclusionsThere is an association between the SNP(rs2254298) of OXTR and childhood autism. Related Articles | Metrics

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Analysis of modern Chinese Medicine Syndrome literatures about cerebral palsy in children by bibliometric methods HUANG Wei，WANG Xuefeng 2010, 2 (1):  15-17.  doi: 10.3969/j.issn.1674-3865.2010.01.006 Abstract ( 434 )   PDF (324KB) ( 106 )   Save ObjectiveUse bibliometric methods to analyze the characteristics of TCM literatures(including the combination of TCM with Western medicine literatures) about cerebral palsy in children in order to summarize the regular patterns to guide clinical and scientific research. MethodsEstablish the inclusion criteria,exclusion criteria and search strategy of the literatures.Search modern literatures of cerebral palsy in children throughout the Online Chinese Biomedical Literature Database,CNKI and CQVIP from January 1,1994 to May 31,2008.Conduct bibliometric analysis on the quantity of the documents,geographical distributions,research orientations and periodical distributions based on the 677 domestic TCM literatures(including the combination of TCM with Western medicine literatures) published within 15 years in China. ResultsThe number of modern TCM literatures showed a risen trend.Henan,Guangdong,Jiangsu,Hunan,Shanghai,Shandong,Liaoning,Fujian,Beijing,and Hubei led the development.The treatments had mainly based on TCM acupuncture and manipulation.The researches had mainly based on acupuncture and the combination of TCM with Western medicine rehabilitation methods.But studies on early interventions and other means of adjuvant therapy still needed to be strengthened.The documents had mainly been published in rehabilitation,acupuncture,neurological diseases and the combination of TCM with Western medicine journals. ConclusionsThe level of domestic studies on cerebral palsy in children are improving continuously.Hot research areas and key research orientations have emerged.But the innovations need to be improved. Related Articles | Metrics

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Retrospective survey of motor function and prognosis of dyskinetic cerebral palsy QIAN Xuguang,LIU Zhenhuan. 2010, 2 (1):  18-20.  doi: 10.3969/j.issn.1674-3865.2010.01.007 Abstract ( 283 )   PDF (332KB) ( 84 )   Save ObjectiveTo analyze the motor function and prognosis of different subtypes of dyskinetic cerebral palsy. MethodsA retrospective survey of 46 cases of dyskinetic cerebral palsy who were treated in Nanhai Affiliated Maternity and Children Hospital of Guangzhou TCM University from January 2005 to October 2009 was carried out.Based on the clinical characteristics,all the patients were divided into 3 types:dystonic type,choreaathetoid type and athetoidspastic type.Files of GMFCS classification and GMFM88 assessment were collected and analyzed. ResultsThere was significant difference of GMFCS levels among the 3 types.Choreaathetoid type had the highest level and the athetoidspastic type had the lowestF=71.596,P<0.001).Each functional area of GMFM88 scale in athetoidspastic type was significantly lower than that of the Choreaathetoid type(P<0.001).The scale of standing position,walking,running and jumping area of dystonic type was significantly lower than that of Choreaathetoid type(P<0.001). ConclusionsMost children of Choreaathetoid type can obtain their own walking ability before 4 years old.Some children of dystonic type can obtain their own walking ability.Children of athetoidspastic type have significant motor delay compared with other types and have difficulty in obtaining their own walking ability Related Articles | Metrics

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Clinical observation of the therapeutic effect of vibroacoustic therapy in relieving spasticity for children with cerebral palsy LIU Zhenhuan,ZHANG Lihong,YIN Xiantao,et al. 2010, 2 (1):  27-29.  doi: 10.3969/j.issn.1674-3865.2010.01.011 Abstract ( 337 )   PDF (392KB) ( 92 )   Save ObjectiveTo observe the curative effect of vibroacoustic therapy in relieving spasticity for children with cerebral palsy. MethodsA total of 36 cases of cerebral palsy were treated by vibroacoustic therapy and listened to the Jiao Music for 30 minutes.We measured the adductor angle and popliteal fossa angle and foot dorsiflexion angle before and after treatment for three times,calculating an average figure and evaluating muscular tension in addition,and calculated the CSS. ResultsThe score of the Cars measurement was obviously different before and after the vibroacoustic therapy（P<0.05），and the angles of most of the children were impoved after the treatment. ConclusionsVibroacoustic therapy can obviously relieve the spasticity for children with cerebral palsy,accordingly improve their limb functions. Related Articles | Metrics

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Clinical trial of parents' role in rehabilitation of children with cerebral palsy YAO Xianhua. 2010, 2 (1):  30-31.  doi: 10.3969/j.issn.1674-3865.2010.01.012 Abstract ( 169 )   PDF (200KB) ( 51 )   Save ObjectiveTo search for the best methods of the rehabilitation while reducing the family burden. MethodsUnder the same conditions(therapists treatment,age and severity of CP)，observe different efficacy on the rehabilitation children with parents involved in the training in different time. ResultsThe total effective rate in children with mild CP was 100%.The total effective rate in children with moderate CP was 97%.The total effective rate in children with severe CP was 80%.The parents to participate in long training time was better than a short time. ConclusionsThe longer the parents participate in the rehabilitation training, the better the clinical result is. Related Articles | Metrics

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The preventive effect of antiasthma particles on inflammatory airway damage of model rats with asthma in remission stage Xing Xianghui，Chen Lu，Song HuiXiao. 2010, 2 (1):  49-51.  doi: 10.3969/j.issn.1674-3865.2010.01.021 Abstract ( 247 )   PDF (366KB) ( 94 )   Save ObjectiveTo discuss the mechanism of antiasthma particles on preventing inflammatory airway damage in asthma remission stage. MethodsProduce rat model of asthma in remission stage to observe the impact of the antiasthma particles concentrate on the serum IL4,EOS,and IgE of the model after gastric perfusion.Compare it with Guben Kechuan Tablets and ketotifen positive controlled groups. ResultsAntiasthma particles could reduce levels of serum EOS,inflammatory cytokines IL4 and immune factors IgE in model rats with asthma in remission stage，and had statistically meaning compared with the positive controlled groups（P<0.05）. ConclusionsAntiasthma particles can inhibit airway inflammation and reduce airway hyperresponsiveness in the model rats,so it has good preventive effect on inflammatory airway damage in model rats with asthma in remission stage. Related Articles | Metrics