Abstract:

Objective：To observe the effect of 10% Li-Pi Compound Preparation and zinc sulfate drug-containing serum on the proliferation of colonic smooth muscle cells(CSMCs) and their regulation effect on braingut peptide, and to explore the mechanism of the two drugs in the treatment of anorexia.Methods：Among the 60 young rats, the CSMCs of 10 rats were cultured, and the remaining 50 rats were randomly divided into five groups: the control group, the low，medium and high-dose Li-Pi Compound Preparation group and the zinc sulfate group, with 10 rats in each group. The control group was given equal volume of drinking water by gavage. The zinc sulfate group was given 20 mg/kg zinc sulfate oral solution by gavage. The low，medium and high-dose groups were given by gavage 2.4, 4.8 and 9.8 g/kg Li-Pi CompoundPreparations respectively. Each group was given gavage once a day for 7 consecutive days, and blood samples were collected to prepare drug-containing serum at 2h after the last gavage.The drug-containing serum of each group was diluted to 10% and was given to rat CSMCs at 9 time points. The effect of drug-containing serum in each group on the proliferation of colonic smooth muscle cells was detected by CCK-8;Western Bolt, ELISA and RT-PCR were used to detect the relative expression levels of LP,NPY and CCK-8 protein concentration in the cells at the optimal starting point of CSMCs proliferation.Results：The 10% different dosedrug-containing serum of Li-Pi Compound Preparations and zinc sulfate could promote the proliferation of CSMCs in rats, and the optimal starting time of proliferation was 24h.Compared with the control group, each drug-containing serum group could increase the concentration of LP and NPY protein in rat CSMCs and the relative expression level of protein and gene（P<0.05),reduce the concentration and the relative expressionlevel of CCK-8 protein（P<0.05).Conclusion：Li-Pi Compound Preparation and zinc sulfate can promote the proliferation of CSMCs,and affect the expression of LP,NPY and CCK-8 in CSMCs, which may be one of the important mechanisms in the treatment of anorexia.

Key words: Anorexia, Li-Pi Compound Preparation, Zinc sulfate, Colonic smooth muscle cells, LP, NPY, CCK-8