ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2018, Vol. 10 ›› Issue (4): 340-343.doi: 10.3969/j.issn.1674-3865.2018.04.020

• 临床研究 • 上一篇    下一篇

儿童哮喘发作期血清调节性T细胞和辅助性T细胞表达分析

郝亮亮，赵雅娟，杨晓彤，李哲   

  1. 110032 沈阳，沈阳市儿童医院内五病房
  • 出版日期:2018-08-25 发布日期:2018-12-03
  • 通讯作者: 赵雅娟，E-mail：116021360@qq.com
  • 作者简介:郝亮亮（1984-），女，医学硕士，主治医师。研究方向：儿童呼吸系统疾病的诊治

Analysis of expression level of peripheral regulatory T cells and helper T cells in asthma children at stage of attack

HAO Liangliang，ZHAO Yajuan，YANG Xiaotong，LI Zhe   

  1.  Neurology Department, the Children's Hospital of Shenyang, Shenyang 110032，China
  • Online:2018-08-25 Published:2018-12-03

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摘要：
目的
分析儿童哮喘发作期血清调节性T细胞和辅助性T细胞的表达。
方法
选择2016年10月至12月沈阳市儿童医院收治的支气管哮喘患儿55例为研究对象，根据临床表现所属的中医证候类型分为寒哮证组26例和热哮证组29例。同期选取参加体检结论健康儿童为对照组28例。3组对象均接受血清调节性T细胞（CD4 CD25+ Treg和CD4+ CD25+ Foxp3+ Treg）和辅助性T细胞（CD4+、CD8+、CD4+/CD8+）表达水平测定。
结果
寒哮证组和热哮证组血清CD4 CD25+ Treg和CD4+ CD25+ Foxp3+ Treg浓度显著低于对照组，寒哮证组血清CD4 CD25+ Treg和CD4+ CD25+ Foxp3+ Treg浓度显著低于热哮证组，差异均有统计学意义（P<0.05）。寒哮证组和热哮证组血清CD4+浓度显著高于对照组，而血清CD8+浓度及CD4+/CD8+比值均显著低于对照组；寒哮证组血清CD4+和CD8+浓度显著低于热哮证组，差异均有统计学意义（P<0.05）。
结论
寒哮证和热哮证患儿均存在明确的血清调节性T细胞和辅助性T细胞表达异常，且以寒哮证病患儿更为显著。

关键词: 支气管哮喘, 寒, 热哮症, 调节性T细胞, 辅助性T细胞, 儿童

Abstract:
Objective
To analyze the expression levels of peripheral regulatory T cells and helper T cells in asthma children at stage of attack.
Methods
Totally 55 children with bronchial asthma treated in Shenyang Children's Hospital from Oct. 2016 to Dec. 2016 were chosen as the research subjects. According to the TCM syndrome type, they were divided into two groups: 26 in cold type asthma group and 29 in heat type asthma group. Another 28 children who were healthy on the physical examination during the same period were included as the control group. All the three groups of children received determination of the expression level of regulatory T cells(CD4 CD25+ Treg and CD4+ CD25+ Foxp3+ Treg) and helper T cells(CD4+,CD8+ and CD4+/CD8+).
Results
CD4 CD25+ Treg and CD4+ CD25+ Foxp3+ Treg expression level of peripheral blood in cold and heat type asthma group was significantly lower than that in the control group. At the same time, CD4 CD25+ Treg and CD4+ CD25+ Foxp3+ Treg expression level of peripheral blood in cold type asthma group was significantly lower than that in heat asthma group(P<0.05). CD4+ expression level of peripheral blood in cold and heat type asthma group was significantly higher than that in the control group, but CD8+ expression level and CD4+/CD8+ ratio of peripheral blood were significantly lower than those in the control group. At the same time, CD4+ and CD8+ expression level in cold type asthma  group was significantly lower than that in the heat type asthma group(P<0.05).
Conclusion
Regulatory T cells and helper T cells of peripheral blood have abnormal expression in cold and heat type asthma children，and the abnormal expression becomes more obvious in the cold type asthma group.

Key words: Bronchial asthma, Cold and heat type asthma, Regulatory T cells, Helper T cell, Child