关键词: 支气管肺炎, 痰培养, 标本采集, 采集时间, 采集方法, 标本质量, 精准度, 儿童

Abstract: Objective To study the influence of different time and method of collecting specimen in sputum culture on the detection quality of specimen in children with bronchopneumonia. Methods A total of 150 children with bronchopneumonia were treated in our hospital from Feb. 2017 and Feb. 2019, and they were divided into control group(75 children) and observation group(75 children) by lottery. The specimen was taken by way of sputum aspiration in the observation group: the first time was on admission to hospital and the second time was at 2h fasting on the morning of the second day after admission; in the control group, the specimen was taken by cotton swab: the first time was right after admission to hospital and the second time was at 2h fasting on the morning of the second day after admission. The total number of strains detected out, the positive rate of strains, the return rate of specimen, and the accuracy rate were compared between the two groups. Results The total detection number and the positive rate of strains in the sputum specimens taken at different time points were higher in observation group than in control group, and the total detection number and positive rate on getting up were higher than at random time points in both groups, the difference being statistically significant(P<0.05); the return rate at different time points was significantly lower in observation group than in control group, and the rate on getting up was lower than at random time points in both groups(P<0.05); the accuracy rate was higher in observation group than in control group at different time points, and it was higher on getting up than at random time points in both groups(P<0.05).ConclusionThe collection of sputum specimen by way of sputum aspiration at fasting on the morningof the second day after admission can significantly increase the detection rate, the positive rate and the accu- racy rate in children with bronchopneumonia , while reducing the return rate; the effect is significant and it is worthy of promotion.

Key words: Bronchopneumonia, Sputum culture, Specimen collection, Collection time, Collection method, Specimen quality, Precision, Children