基于文献研究的中国孤独症儿童肠道菌群特征分析

doi:10.20274/j.cnki.issn.2025.04.006

Abstract

Abstract:

Objective To review the characteristics of intestinal microbiome in children with autism spectrum disorder (ASD) based on existing literature evidence, and to explore their correlation with core symptoms of ASD, in order to provide theoretical basis for intestinal microbiome intervention in ASD. Methods The literature on intestinal microbiome characteristics of children with ASD in China was systematically searched for in Wanfang, China national knowledge infrastructure (CNKI), Weipu database (VIP), Web of Science and other relevant databases until January 6, 2024. Qualified articles were selected according to inclusion and exclusion criteria, data were extracted, and the quality of the included articles was evaluated using the Newcastle and Ottawa scale (NOS). Results A total of 33 articles were included, and the study subjects were 2 042 children with ASD aged 2 to 14 years.In 16 studies α or β diversity in the intestinal microbiome was analyzed in the children with ASD and healthy children, of which seven reported α and β diversity, five described only α diversity, and four mentioned only β diversity, but the results were inconsistent.At the phylum level,Firmicutes were the most frequently reported, with 9 articles mentioning that their abundance was related to ASD, followed by Bacteroidetes and Proteobacteria, each mentioned in 7 articles. Megamonas, Megasphaera, Barnesiella, Escherichia, Streptococcus and Bifidobacterium were associated with the severity of ASD. Megamonas, Blautia, Streptococcus, Bacteroides, Clostridium, Sutterella, Lactobacillus and Bifidobacterium were reported to be associated with social behavior, while Megamonas was associated with speech disorders. Bifidobacterium, Streptococcus, Blautia and Bacteroides were associated with cognitive development. Clostridium, Lactobacillus and Bifidobacterium were associated with stereotypical behavior, while Bifidobacterium was associated with mood disorders. The abundance of Firmicutes, Bacteroidetes and Bacteroides was associated with gastrointestinal symptoms such as constipation. Conclusion There are significant differences in intestinal microbiome between children with ASD and healthy children. There is a great number of intestinal microbiome involved in the core and accompanying symptoms of ASD, and most of the microbiome associated with ASD belong to phylum Firmicutes, indicating that the abundance of Firmicutes is closely related to the occurrence and development of ASD.

Key words: Autism spectrum disorder, Intestinal microbiome, Systematic review, Chinese children

CLC Number:

R277.7

Xingqian YI, Jing Ying NG, Zhiyi XIONG, Xiaofan CHEN, Ying LIU, Xiaoyan LI, Li WANG. Analysis of intestinal microbiome characteristics of children with autism in China based on literature research[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2025, 17(4): 302-312.

Figures/Tables 4

References 93

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项目	结果	总数	研究文献
α多样性	↑	2	[30,34]
	↓	5	[15,31,33,36,42]
	-	5	[12,21,23,32,40]
β多样性	*	9	[13,18,23,29-32,40,42]
	-	2	[12,38]

菌群分类	总数	丰度升高	丰度降低
厚壁菌门	9	5^{[14,19,29-30,38]}	4^{[17,24,31,37]}
拟杆菌门	7	1^[37]	6^{[14,19,21,29-30,38]}
变形菌门	7	4^{[14,19,35,38]}	3^[11,21,37]
放线菌门	5	4^{[15,22-23,29]}	1^[38]
酸杆菌门	3	1^[31]	2^[17,24]

Analysis of intestinal microbiome characteristics of children with autism in China based on literature research

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[4]	CHEN Xi, PENG Yaqi, LI Wei, YANG Hongsheng, WANG Junhong. A brief analysis of the pathogenesis and syndrome differentiation of autism spectrum disorders from the "brain-spleen and kidney correlation" [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2024, 16(1): 88-92.
[5]	GUO Zhiyi, YANG Kejia, LEI Jing, ZENG Yongjian, GUO Zhidong, SUN Xiangjuan. Risk factors influencing the prognosis of tic disorders in Chinese children: a systematic review [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2023, 15(4): 283-290.
[6]	LIU Annan, JIANG Zhimei. Research progress of complementary and alternative medicine intervention in autism spectrum disorders [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2023, 15(1): 34-40.
[7]	WU Jianye, XU Lei, KANG Beibei, JIANG Zhimei, XU Ruiyang, CHEN Ning. A study on the mechanism of short-chain fatty acids produced by the intestinal flora affecting the core symptoms of autism spectrum disorder [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2022, 14(6): 481-485.
[8]	LYU Nan, YANG Yang, SHANG Qing, WAN Kai, MA Caiyun. Effect of sensorimotor training on motor skills of children with autism spectrum disorder [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2022, 14(1): 37-41.
[9]	ZHANG Xiaoyu, ZHANG Yanlei. Effect of sensory integration training based on the Denver model on the communication ability of children with autism spectrum disorder aged 2 to 5 years and parenting stress [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2022, 14(1): 41-44.
[10]	ZHAN Hong, BAI Shuxia, WANG Jintang. Research progress in the pathogenesis of autism spectrum disorders in children [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(2): 112-.
[11]	TAN Yaqiong, HU Jihong, LUO Wei, WANG Paoqiu, LI Haixia. Analysis of vitamin D and water-soluble vitamin levels in children with autism spectrum disorder [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2020, 12(5): 395-399.
[12]	DONG Chongkai, ZHANG Jihua, GAO Bingnan, GE Xiao, JIANG Guo, ZHAO Yingying. Efficacy of repetitive transcranial magnetic stimulation(TMS) in the treatment of children with autism spectrum disorders aged 2-6 years [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2020, 12(3): 209-211.
[13]	ZHAO Mengxia, SONG Zhenzhen, YU Xiaojia, ZHANG Weidan. Effects of group language training on children with autism spectrum disorders [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2020, 12(3): 212-214.
[14]	MA Zongyan, WANG Suli, DIAO YIingying, CHEN Jiangui, CHAI Anbo. Factors affecting rehabilitation compliance of children with autism spectrum disorder [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2020, 12(2): 135-137.
[15]	HE Jinhua, WANG Paoqiu, QIN Rong, YUAN Liping, LIU Zhixiong, LIU Hongwen.. Effect of Huxiang compatibility of five meridians combined with acupuncture on language ability of children with autism spectrum disorder [J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2019, 11(6): 487-491.